AIF anticorps

N° du produit ABIN4953229

L’anticorps anti-AIF Monoclonal Souris est utilisé pour la détection de AIF dans des échantillons de Humain. Il a été validé pour WB, IF et ICC.

Aperçu rapide pour AIF anticorps (ABIN4953229)

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AIF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 8H1-B10-A12

Détail du produit anti-AIF anticorps

Purification: Protein G affinity

Immunogène: A human recombinant partial protein was used as the immunogen for this AIF antibody.

Isotype: IgG2a

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the AIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,ICC/IF: 1:200

Restrictions: For Research Use only

Stockage

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Stock: -20 °C

Stockage commentaire: Store the AIF antibody at -20°C.

Détails sur AIF

Antigène: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Autre désignation: AIFM1 / AIF / Apoptosis-Inducing Factor

Sujet: Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]

UniProt: O95831

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, L'effet Warburg