BTK anticorps (AA 396-430)

N° du produit ABIN3030208

Cet anticorps Lapin Polyclonal détecte spécifiquement BTK dans WB et ELISA. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour BTK anticorps (AA 396-430) (ABIN3030208)

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BTK est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-BTK anticorps

Épitope: AA 396-430

Réactivité croisée (Details): Expected species reactivity: Chicken

Purification: Antigen affinity purified

Immunogène: This BTK antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 396-430 amino acids from the Central region of human BTK.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the BTK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the BTK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur BTK

Antigène: BTK (Bruton Agammaglobulinemia tyrosine Kinase (BTK))

Autre désignation: BTK

Sujet: Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling. Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation. After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members. PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK. BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways. Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway. The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense. Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells. Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation. BTK plays also a critical role in transcription regulation. Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes. BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B. Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR. GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression. ARID3A and NFAT are other transcriptional target of BTK. BTK is required for the formation of functional ARID3A DNA-binding complexes. There is however no evidence that BTK itself binds directly to DNA. BTK has a dual role in the regulation of apoptosis.

UniProt: Q06187

Pathways: Fc-epsilon Receptor Signaling Pathway, Hormone Transport, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, BCR Signaling