ErbB2/Her2 anticorps (pTyr1140)

N° du produit ABIN3029502

Cet anticorps anti-ErbB2/Her2 Polyclonal Lapin (ABIN3029502) détecte spécifiquement ErbB2/Her2 dans WB, ELISA et DB. L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour ErbB2/Her2 anticorps (pTyr1140) (ABIN3029502)

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ErbB2/Her2 est non-conjugé

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Détail du produit anti-ErbB2/Her2 anticorps

Épitope: pTyr1140

Fonction: HER2 Antibody / ErbB2 (pY1140)

Purification: Antigen affinity purified

Immunogène: This phospho-ErbB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1140 of mouse HER2/ERBB2.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the Phospho-ErbB2 (pY1140) Antibody / Signaling Marker may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles

Stock: -20 °C

Stockage commentaire: Aliquot the phospho-ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur ErbB2/Her2

Antigène: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Autre désignation: Erbb2

Sujet: ErbB2 receptor tyrosine kinase 2 (ERBB2), also known as HER2, is a transmembrane receptor that regulates cell proliferation, survival, and differentiation through activation of intracellular signaling pathways. Phospho-ErbB2 (pY1140) antibody, also referred to as phospho-HER2 antibody and phospho-ERBB2 antibody in the literature, detects phosphorylation at tyrosine 1140, a residue located within the intracellular domain of HER2 that contributes to downstream signaling activity.

Phosphorylation of HER2 occurs at multiple tyrosine residues following receptor activation, forming a coordinated signaling network that governs pathway engagement. Y1140 is positioned within this network and participates in transmission of signals to downstream pathways such as PI3K/AKT and MAPK. While not as extensively characterized as major activation sites, phosphorylation at Y1140 reflects receptor signaling activity and contributes to propagation of intracellular responses following receptor engagement.

HER2 signaling is a major driver of tumor biology, particularly in cancers characterized by ERBB2 amplification such as breast carcinoma. In these settings, phosphorylation at residues including Y1140 is associated with active signaling states that support tumor cell growth and survival. Detection of phosphorylation at this site provides insight into receptor activity within the broader signaling cascade rather than serving as a primary activation marker alone.

Y1140 functions within a phosphorylation cluster that includes nearby residues such as Y1112, Y1139, Y1221, Y1222, and Y1248. Together, these sites regulate the initiation, propagation, and integration of HER2-mediated signaling. Analysis of phosphorylation at Y1140 complements detection of these other residues and supports a more comprehensive understanding of HER2 signaling dynamics.

Phospho-specific detection of ERBB2 at Y1140 enables investigation of receptor signaling beyond primary activation events, supporting studies focused on pathway activity, signal propagation, and cellular response mechanisms in both normal and disease contexts.

For detection of activated HER2 phosphorylation, see our < a href=../tds/p-erbb2-antibody-py1248-f48395>HER2 phospho antibody (pY1248).

UniProt: P70424

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development