HNRNPF anticorps (N-Term)

N° du produit ABIN497944

Cet anticorps Lapin Polyclonal détecte spécifiquement HNRNPF dans WB, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat et Boeuf (Vache).

Aperçu rapide pour HNRNPF anticorps (N-Term) (ABIN497944)

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivité: Humain, Souris, Rat, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HNRNPF est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-HNRNPF anticorps

Épitope: AA 1-164, N-Term

Specificité: Recognizes hnRNP-F/HNRNPF (1-174).
Cellular Localization: Nucleus/nucleoplasm

Réactivité croisée (Details): Species reactivity (expected):Mouse (98 %), Rat (83 %), Bovine (97 %).
Species reactivity (tested):Human.

Purification: Antigen-Affinity Chromatography.

Immunogène: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of hnRNP-F

Information d'application

Indications d'application: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100-1/250. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.72 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.

Agent conservateur: Thimerosal (Merthiolate)

Précaution d'utilisation: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at -20 °C.

Détails sur HNRNPF

Antigène: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Autre désignation: HnRNP-F / HNRNPF

Sujet: HnRNP-F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.Synonyms: HNRPF, Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1

ID gène: 3185, 9606

UniProt: P52597