SIAH1 anticorps

N° du produit ABIN498346

Cet anticorps Lapin Polyclonal détecte spécifiquement SIAH1 dans WB, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain, Rat et Souris.

Aperçu rapide pour SIAH1 anticorps (ABIN498346)

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-SIAH1 anticorps

Specificité: This antibody detects endogenous levels of Siah-1/2 protein.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat.
Also reacts to: SIAH2

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1

Sujet: SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a

Poids moléculaire: approx. 40.0 kDa

ID gène: 6477

NCBI Accession: NP_001006611

UniProt: Q8IUQ4