SIAH1 anticorps

N° du produit ABIN498346

Détail du produit anti-SIAH1 anticorps

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIAH1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of Siah-1/2 protein.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat.
Also reacts to: SIAH2

Purification: Affinity Chromatography using epitope-specific immunogen

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SIAH1

Antigène: SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))

Autre désignation: SIAH1 (SIAH1 Produits)

Synonymes: anticorps SIAH, anticorps Siah1, anticorps Siah1a, anticorps id:ibd2635, anticorps zgc:56026, anticorps SIAH1A, anticorps siah, anticorps E3 ubiquitin-protein ligase Siah1, anticorps E3 ubiquitin-protein ligase siah-1, anticorps siah E3 ubiquitin protein ligase 1, anticorps siah E3 ubiquitin protein ligase 1 L homeolog, anticorps Tsp_03055, anticorps siah-1, anticorps Siah1, anticorps siah1, anticorps SIAH1, anticorps siah1.L

Sujet: SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.Synonyms: E3 ubiquitin-protein ligase SIAH1, HUMSIAH, Seven in absentia homolog 1, Siah-1, Siah-1a

Poids moléculaire: approx. 40.0 kDa

ID gène: 6477

NCBI Accession: NP_001006611

UniProt: Q8IUQ4