PIK3R1 anticorps (pTyr199, pTyr458)

N° du produit ABIN498695

L’anticorps Lapin Polyclonal anti-PIK3R1 a été validé pour WB, IF et IHC (p). Il convient pour détecter PIK3R1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PIK3R1 anticorps (pTyr199, pTyr458) (ABIN498695)

Antigène: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIK3R1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PIK3R1 anticorps

Épitope: pTyr199, pTyr458

Specificité: This antibody detects endogenous levels of p85a/p55 only when phosphorylated at Tyr467/Tyr199.

Réactivité croisée (Details): Species reactivity (tested):Human, Mouse, Rat.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PIK3R1

Antigène: PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))

Autre désignation: PIK3R1 / GRB1

Sujet: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulinstimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.Synonyms: PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha

Poids moléculaire: approx. 55, 85 kDa

ID gène: 5295

NCBI Accession: NP_852556

UniProt: P27986

Pathways: TCR Signaling, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, L'effet Warburg