CPI-17 anticorps (pThr38)

N° du produit ABIN498756

Détail du produit anti-CPI-17 anticorps

Antigène: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Épitope: pThr38

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CPI-17 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of CPI-17 protein only when phosphorylated at Thr38.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CPI-17

Antigène: CPI-17 (PPP1R14A) (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 14A (PPP1R14A))

Autre désignation: PPP1R14A (PPP1R14A Produits)

Synonymes: anticorps MGC81874, anticorps cpi-17, anticorps ppp1inl, anticorps CPI-17, anticorps im:7147597, anticorps ppp1r14a, anticorps zgc:110700, anticorps PPP1R14A, anticorps CPI17, anticorps PPP1INL, anticorps Cpi17, anticorps 1110001M11Rik, anticorps protein phosphatase 1 regulatory inhibitor subunit 14A L homeolog, anticorps protein phosphatase 1 regulatory inhibitor subunit 14A, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 14Aa, anticorps protein phosphatase 1, regulatory (inhibitor) subunit 14A, anticorps ppp1r14a.L, anticorps ppp1r14a, anticorps ppp1r14aa, anticorps PPP1R14A, anticorps Ppp1r14a

Sujet: CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle Myosin phosphate. CPI-17 was originally identified as a 17 kDa PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonin 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle cont-raction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.Synonyms: 17 kDa PKC-potentiated inhibitory protein of PP1, CPI-17, CPI17, PPP1INL, Protein phosphatase 1 regulatory subunit 14A

Poids moléculaire: approx. 22 kDa

ID gène: 94274

NCBI Accession: NP_150281

UniProt: Q96A00