PAK2 anticorps

N° du produit ABIN499019

Détail du produit anti-PAK2 anticorps

Antigène: PAK2 (P21-Activated Kinase 2 (PAK2))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAK2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of PAKγ protein. (region surrounding Arg186)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PAK2

Antigène: PAK2 (P21-Activated Kinase 2 (PAK2))

Autre désignation: PAK2 (PAK2 Produits)

Synonymes: anticorps PAK65, anticorps PAKgamma, anticorps gamma-PAK, anticorps 5330420P17Rik, anticorps A130002K10Rik, anticorps AI836325, anticorps D16Ertd269e, anticorps PAK-2, anticorps mKIAA4182, anticorps pak2, anticorps cb422, anticorps kinase, anticorps zgc:91798, anticorps wu:fb71h01, anticorps si:dz198m22.1, anticorps pak65, anticorps pakgamma, anticorps PAKI, anticorps Pak3, anticorps CH73-159L23.1, anticorps wu:fc52b03, anticorps zgc:113857, anticorps p21 (RAC1) activated kinase 2, anticorps p21 protein (Cdc42/Rac)-activated kinase 2, anticorps p21 protein (Cdc42/Rac)-activated kinase 2a, anticorps p21 protein (Cdc42/Rac)-activated kinase 2 S homeolog, anticorps p21 protein (Cdc42/Rac)-activated kinase 2b, anticorps PAK2, anticorps Pak2, anticorps pak2a, anticorps pak2.S, anticorps pak2b

Sujet: Three isoforms of serine/threonine kinases, designated 7PAK p68, 8PAK p65 and 9PAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The 7, 8, and 9 PAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on 9 PAK, including Ser 19, Ser 141 Thr 402, phosphorylation of Ser 141 and Thr 402 is correlated with 9 PAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates. One such putative substrate is Mek kinase, an upstream effector of Mek4, which is involved in the JNK signaling pathway. While the PAK isoforms interact in a GTP dependent manner with Rac 1 and Cdc 42, they do not interact with Rho.Synonyms: Gamma-PAK, PAK 2, PAK gamma, PAK-2, PAK65, Serine/threonine-protein kinase PAK 2, p21-activated kinase 2, p58

Poids moléculaire: approx. 58 kDa

ID gène: 5062

NCBI Accession: NP_002568

UniProt: Q13177

Pathways: Signalisation MAPK, Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha