Nephrin anticorps (C-Term)

N° du produit ABIN500330

Détail du produit anti-Nephrin anticorps

Antigène: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Nephrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificité: This antibody detects Nephrin.

Purification: Peptide affinity chromatography

Immunogène: Nephrin antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human Nephrin.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % Sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Nephrin (ABIN500330)

Gu, Zhou, Noble, Border, Cheung, Huang: "Targeting reduction of proteinuria in glomerulonephritis: Maximizing the antifibrotic effect of valsartan by protecting podocytes." dans: Journal of the renin-angiotensin-aldosterone system : JRAAS, Vol. 15, Issue 2, pp. 177-89, (2015) (PubMed).

Zhang, Gu, Lawrence, Cheung, Huang: "A plasminogen activator inhibitor type 1 mutant retards diabetic nephropathy in db/db mice by protecting podocytes." dans: Experimental physiology, Vol. 99, Issue 5, pp. 802-15, (2014) (PubMed).

Détails sur Nephrin

Antigène: Nephrin (NPHS1) (Nephrosis 1, Congenital, Finnish Type (Nephrin) (NPHS1))

Autre désignation: Nephrin (NPHS1 Produits)

Synonymes: anticorps NPHS1, anticorps nphs1l, anticorps nphs1, anticorps CNF, anticorps NPHN, anticorps nephrin, anticorps NephrinB, anticorps NPHS1, nephrin, anticorps nephrosis 1, congenital, Finnish type (nephrin), anticorps NPHS1 nephrin S homeolog, anticorps nephrin, anticorps NPHS1 nephrin, anticorps nephrosis 1, nephrin, anticorps NPHS1, anticorps nphs1, anticorps nphs1.S, anticorps LOC5579127, anticorps Nphs1

Sujet: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. Despite its predicted molecular weight, Nephrin often migrates at a lower than expected size in SDS-PAGE.Synonyms: NPHN, NPHS1

ID gène: 4868

NCBI Accession: NP_004637

UniProt: O60500

Pathways: Regulation of Actin Filament Polymerization, Skeletal Muscle Fiber Development