PDE5A anticorps (N-Term)

N° du produit ABIN5518785

Cet anticorps Lapin Polyclonal détecte spécifiquement PDE5A dans WB et IHC. Il présente une réactivité envers Humain et Rat.

Aperçu rapide pour PDE5A anticorps (N-Term) (ABIN5518785)

Antigène: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDE5A est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-PDE5A anticorps

Épitope: AA 20-63, N-Term

Fonction: Anti-PDE5A Antibody Picoband®

Séquence: QKQQQRDQDS VEAWLDDHWD FTFSYFVRKA TREMVNAWFA ERVH

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PDE5A Antibody Picoband® (ABIN5518785). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human PDE5A, different from the related mouse sequence by eight amino acids.

Isotype: IgG

Information d'application

Indications d'application: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Gebska, M. A., Stevenson, B. K., Hemnes, A. R., Bivalacqua, T. J., Haile, A., Hesketh, G. G., Murray, C. I., Zaiman, A. L., Halushka, M. K., Krongkaew, N., Strong, T. D., Cooke, C. A., El-Haddad, H., Tuder, R. M., Berkowitz, D. E., Champion, H. C.Phosphodiesterase-5A (PDE5A) is localized to the endothelial caveolae and modulates NOS3 activity. Cardiovasc. Res. 90: 353-363, 2011. 2. Lin, C.-S., Chow, S., Lau, A., Tu, R., Lue, T. F. Human PDE5A gene encodes three PDE5 isoforms from two alternate promoters.Int. J. Impotence Res. 14: 15-24, 2002. 3. Loughney, K., Hill, T. R., Florio, V. A., Uher, L., Rosman, G. J., Wolda, S. L., Jones, B. A., Howard, M. L., McAllister-Lucas, L. M., Sonnenburg, W. K., Francis, S. H., Corbin, J. D., Beavo, J. A., Ferguson, K. Isolation and characterization of cDNAs encodingPDE5A, a human cGMP-binding, cGMP-specific 3-prime,5-prime-cyclic nucleotide phosphodiesterase. Gene 216: 139-147, 1998.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PDE5A

Antigène: PDE5A (phosphodiesterase 5A, cGMP-Specific (PDE5A))

Autre désignation: PDE5A

Sujet:

Synonyms: cGMP-specific 3',5'-cyclic phosphodiesterase,3.1.4.35 ,cGMP-binding cGMP-specific phosphodiesterase,CGB-PDE,PDE5A,PDE5,

Tissue Specificity: Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.

Background: cGMP-specific phosphodiesterase type 5 is an enzyme from the phosphodiesterase class. It is found in various tissues, most prominently the corpus cavernosum and the retina. It has also been recently discovered to play a vital role in the cardiovascular system. Furthermore, PDE5A also plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This PDE5A gene is mapped to 4q26.

Poids moléculaire: 100 kDa

ID gène: 8654

UniProt: O76074

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling