CD3 epsilon anticorps (Internal Region)

N° du produit ABIN5539697

Cet anticorps Chèvre Polyclonal détecte spécifiquement CD3 epsilon dans WB et ELISA. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour CD3 epsilon anticorps (Internal Region) (ABIN5539697)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Western Blotting (WB), ELISA

Classe de qualité: Verified

Détail du produit anti-CD3 epsilon anticorps

Épitope: Internal Region

Fonction: CD3E (cytoplasmic)

Séquence: RKGQRDLYSG LNQR

Specificité: This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.

Réactivité croisée: Humain, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB

Peptide ELISA: antibody detection limit dilution 1:128000.

Commentaires:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3E

Sujet: CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro

ID gène: 916, 315609

NCBI Accession: NP_000724

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway