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PDPK1 anticorps (AA 457-556)

Name: PDPK1 anticorps (AA 457-556)
Brand: antibodies-online
SKU: ABIN5542259
Price: 640.59 EUR
Availability: InStock

Cet anticorps Souris Monoclonal détecte spécifiquement PDPK1 dans WB, ELISA, IHC, ICC et FACS. Il présente une réactivité envers Humain, Souris, Rat et Singe.

Western blot analysis using PDPK1 mAb against human PDPK1 (AA: 457-556) recombinant protein. (PDPK1 anticorps (AA 457-556))

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng), (PDPK1 anticorps (AA 457-556))

ELISA

Western blot analysis using PDPK1 mAb against HEK293 (1) and PDPK1 (AA: 457-556)-hIgGFc transfected HEK293 (2) cell lysate. (PDPK1 anticorps (AA 457-556))

Western blot analysis using PDPK1 mouse mAb against MCF-7 (1), Hela (2), K562 (3), U937 (4), A549 (5), NIH/3T3 (6), Jurkat (7), PC-12 (8), and Cos7 (9) cell lysate. (PDPK1 anticorps (AA 457-556))

Flow cytometric analysis of A549 cells using PDPK1 mouse mAb (green) and negative control (red). (PDPK1 anticorps (AA 457-556))

FACS

Immunofluorescence analysis of A549 cells using PDPK1 mouse mAb (green). (PDPK1 anticorps (AA 457-556))

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using PDPK1 mouse mAb with DAB staining. (PDPK1 anticorps (AA 457-556))

IHC

7 images

N° du produit ABIN5542259

Aperçu rapide pour PDPK1 anticorps (AA 457-556) (ABIN5542259)

Antigène

Voir toutes PDPK1 Anticorps

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reactivité

Humain, Souris, Rat, Singe

Hôte

Souris

Clonalité

Monoclonal

Conjugué

Cet anticorp PDPK1 est non-conjugé

Application

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone

7E4G11

Discover our top product PDPK1 Anticorps primaire

Épitope
23

19

15

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15

15

8

6

6

4

4

4

3

3

3

2

2

2

2

2

2

2

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2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 457-556

Fonction

PDPK1 Antibody

Purification

Purified antibody

Immunogène

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Isotype

IgG1
anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
PDPK1 Reactivité: Humain, Souris, Rat WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 150-429) antibody
PDPK1 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-150) antibody
PDPK1 Reactivité: Humain WB, IF Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 1-429) antibody
PDPK1 Reactivité: Humain WB, PLA Hôte: Souris Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody
PDPK1 Reactivité: Humain, Rat, Singe WB, ELISA, IHC, ICC, FACS Hôte: Souris Monoclonal 4A11 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 524-556), (C-Term) antibody
PDPK1 Reactivité: Humain, Souris, Rat WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 83-338) antibody
PDPK1 Reactivité: Humain WB, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 457-556) antibody
PDPK1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 2E2 unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 277-556) antibody
PDPK1 Reactivité: Humain ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pTyr373), (pTyr376) antibody
PDPK1 Reactivité: Humain, Souris WB, ELISA Hôte: Lapin Polyclonal unconjugated

Indications d'application

ELISA: 1/10000
FCM: 1/200 - 1/400
ICC: 1/50 - 1/200

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Antigène

PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Autre désignation

PDPK1

Sujet

Phosphoinositide-dependent kinase 1 (PDPK1, PDK1) is a serine/threonine protein kinase integral to the function of the PI 3-K/Akt signaling pathway. PDK1 and mTORC2 both phosphorylate and activate PKB/Akt, ensuring a cellular response to stimuli such as growth factors and insulin signaling. Akt is the main effector of PDK1.

Poids moléculaire

63.2 kDa

UniProt

O15530

Pathways

Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals