Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
Séquence
SELDPKGQHV C
Specificité
This antibody is expected to recognize all reported isoforms of SCARF1. Epitope Epitope corresponding to aa 20-31, mapping to the mature Human SCARF1/SREC1 protein.
Réactivité croisée (Details)
Species reactivity (tested):Human.
Purification
Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogène
Peptide with sequence from the internal region of the protein sequence according to NP_003684.2, NP_663325.1, NP_663327.2. Genename: SCARF1
SCARF1
Reactivité: Humain, Souris, Rat
Hôte: Lapin
Polyclonal
Alexa Fluor 680
Indications d'application
Peptide ELISA: 1/64000. Western blot: 0.3 - 1.0 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
anticorps SREC, anticorps SREC-I, anticorps SREC1, anticorps AA986099, anticorps mKIAA0149, anticorps scavenger receptor class F member 1, anticorps scavenger receptor class F, member 1, anticorps SCARF1, anticorps Scarf1
Sujet
SCARF1 is a scavenger receptor that is expressed in endothelial cells and mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Transcription factors ZNF444/EZF-2 and SP1 are responsible for the regulation of this gene. Five alternatively spliced transcript variants encoding distinct isoforms have been described.Synonyms: Acetyl LDL receptor, Endothelial cells scavenger receptor, KIAA0149, SREC, Scavenger receptor class F member 1