ABCC4 anticorps (C-Term)

N° du produit ABIN374484

Détail du produit anti-ABCC4 anticorps

Antigène: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Épitope: C-Term

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp ABCC4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Séquence: CNGQPSTLTI FETAL

Specificité: This antibody antibody is expected to recognize isoform 1 (NP_005836.2) only.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the C-Terminus of the protein sequence (NP_005836.2). Genename: ABCC4

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/64000. Western blot: 1-3 μg/mL. Approx 170 kDa band observed in rat renal cortex membranes(calculated MW of 140 kDa according to Rat NP_596902.1 and 150 kDa according to HumanNP_005836.2). Immunohistochemistry on Paraffin Sections: 2-4 μg/mL. In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flatbasal cells and weak cytoplasmic staining in the columnar secretory cells of theepithelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-ABCC4 (ABIN374484)

Zhang, Dong, Wang, Ma, Wang, Wu: "Stable knock-down of efflux transporters leads to reduced glucuronidation in UGT1A1-overexpressing HeLa cells: the evidence for glucuronidation-transport interplay." dans: Molecular pharmaceutics, Vol. 12, Issue 4, pp. 1268-78, (2016) (PubMed).

Li, Sun, Zhang, Wang, Wu: "Efflux transport of chrysin and apigenin sulfates in HEK293 cells overexpressing SULT1A3: The role of multidrug resistance-associated protein 4 (MRP4/ABCC4)." dans: Biochemical pharmacology, Vol. 98, Issue 1, pp. 203-14, (2016) (PubMed).

Quan, Wang, Dong, Zhang, Wu: "Characterization of chrysin glucuronidation in UGT1A1-overexpressing HeLa cells: elucidating the transporters responsible for efflux of glucuronide." dans: Drug metabolism and disposition: the biological fate of chemicals, Vol. 43, Issue 4, pp. 433-43, (2015) (PubMed).

Gori, Rodriguez, Pellegrini, Achtari, Hornung, Chardonnens, Wunder, Fiche, Canny: "Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4)." dans: Fertility and sterility, Vol. 99, Issue 7, pp. 1965-73.e2, (2013) (PubMed).

Montani, Hermanns, Herrmanns, Müntener, Wild, Sulser, Kristiansen: "Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression." dans: Virchows Archiv : an international journal of pathology, Vol. 462, Issue 4, pp. 437-43, (2013) (PubMed).

Brachs, Lang, Buslei, Purohit, Fürnrohr, Kalbacher, Jäck, Mielenz: "Monoclonal antibodies to discriminate the EF hand containing calcium binding adaptor proteins EFhd1 and EFhd2." dans: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 4, pp. 237-45, (2013) (PubMed).

Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." dans: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

Détails sur ABCC4

Antigène: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Autre désignation: ABCC4 / MRP4 (ABCC4 Produits)

Synonymes: anticorps EST170205, anticorps MOAT-B, anticorps MOATB, anticorps MRP4, anticorps D630049P08Rik, anticorps Mrp4, anticorps ATP binding cassette subfamily C member 4, anticorps multidrug resistance-associated protein 4, anticorps ATP-binding cassette, sub-family C (CFTR/MRP), member 4, anticorps ABCC4, anticorps LOC515333, anticorps LOC616707, anticorps LOC695407, anticorps abcc4, anticorps Abcc4

Sujet: ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MRP4 is a member of the MRP subfamily which is involved in multi-drug resistance. MRP4 may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms.Synonyms: ATP-binding cassette sub-family C member 4, MOAT-B, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B, Multidrug resistance-associated protein 4

Poids moléculaire: 149kDa (Rat NP_596902.1) and 150kDa (Human NP_005836.2).

ID gène: 10257, 9606

UniProt: O15439