SMAD4 anticorps

N° du produit ABIN5557399

L’anticorps Lapin Monoclonal anti-SMAD4 a été validé pour WB, IHC (p) et IF (cc). Il convient pour détecter SMAD4 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour SMAD4 anticorps (ABIN5557399)

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp SMAD4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 3A1

Détail du produit anti-SMAD4 anticorps

Réactivité croisée: Humain, Souris, Rat

Purification: Purified by Protein A.

Immunogène: Recombinant human Smad4 protein, around N-terminal 1-200aa.

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Détails sur SMAD4

Antigène: SMAD4 (SMAD Family Member 4 (SMAD4))

Autre désignation: Smad4

Sujet:

Synonyms: JIP, DPC4, MADH4, MYHRS, Mothers against decapentaplegic homolog 4, MAD homolog 4, Mothers against DPP homolog 4, Deletion target in pancreatic carcinoma 4, SMAD family member 4, SMAD 4, Smad4, hSMAD4

Background: In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site, required for syngernistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

ID gène: 4089

UniProt: Q13485

Pathways: Cycle Cellulaire, Chromatin Binding, Autophagy