YY1 anticorps (AA 249-414)

N° du produit ABIN5563972

Cet anticorps anti-YY1 Polyclonal Lapin (ABIN5563972) détecte spécifiquement YY1 dans WB, IHC, IP, ChIP-seq et CUT&Tag. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour YY1 anticorps (AA 249-414) (ABIN5563972)

Antigène: YY1 (YY1 Transcription Factor (YY1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp YY1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Tagmentation (CUT&Tag)

Détail du produit anti-YY1 anticorps

Épitope: AA 249-414

Fonction: YY1 antibody (pAb)

Purification: Affinity Purified

Immunogène: This antibody was raised against a recombinant protein corresponding to amino acids 249-414 of human YY1.

Isotype: IgG

Information d'application

Indications d'application: ChIP-Seq: 10 µL per ChIP IP: 0.5 - 4 µL per IP WB*: 1:1,000 - 1:5,000 dilution IHC (FFPE): 1:1,000 dilution CUT&RUN: 1 µL per 50 µL reaction *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détails sur YY1

Antigène: YY1 (YY1 Transcription Factor (YY1))

Autre désignation: YY1

Sujet: YY1 (Yin Yang 1) is a transcription factor that belongs to the GLI-Kruppel class of zinc finger transcription factors and is ubiquitously expressed. YY1 is known to have a fundamental role in normal biologic processes such as embryogenesis, differentiation, replication and cellular proliferation. YY1 exerts its effects on genes involved in these processes via its ability to initiate, activate or repress transcription depending upon the context in which it binds. Since expression and function of YY1 are known to be intimately associated with progression through phases of the cell cycle, the physiologic significance of YY1 activity has been applied to models of tumor biology. It can repress and activate a number of gene promoters and direct histone deacetylases (HDACs) and histone acetyltransferases (HATs).

Poids moléculaire: 63 kDa

NCBI Accession: NP_003394