D Amino Acid Oxidase anticorps (HRP)

N° du produit ABIN5596780

Détail du produit anti-D Amino Acid Oxidase anticorps (HRP)

Antigène: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp D Amino Acid Oxidase est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.

Purification: D-Amino Acid Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].

Immunogène:

Immunogen: D-Amino Acid Oxidase [Pig Kidney]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-D-Amino Acid Oxidase has been assayed against 1.0 μg of D-Amino Acid Oxidase [Pig Kidney] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:12,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur D Amino Acid Oxidase

Antigène: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Autre désignation: D-Amino Acid Oxidase (DAO Produits)

Synonymes: anticorps DAAO, anticorps DAMOX, anticorps OXDA, anticorps daao, anticorps oxda, anticorps damox, anticorps DAO, anticorps Dao1, anticorps DKFZp469N2232, anticorps AI987963, anticorps Dao-1, anticorps SC5F2A.23c, anticorps wu:fb55b11, anticorps zgc:113922, anticorps ABP, anticorps ABP1, anticorps DAO1, anticorps KAO, anticorps MGC114783, anticorps Abp, anticorps Abp1, anticorps D-amino acid oxidase, anticorps D-amino-acid oxidase, anticorps D-amino-acid oxidase, tandem duplicate 1, anticorps amine oxidase, copper containing 1, anticorps D-amino-acid oxidase L homeolog, anticorps DAO, anticorps Dao, anticorps dao, anticorps SCO6740, anticorps dao.1, anticorps AOC1, anticorps PTRG_02224, anticorps SJAG_02496, anticorps dao.L, anticorps Aoc1

Sujet:

Synonyms: D-amino-acid oxidase DAMOX DAAO DAO EC=1.4.3.3

Background: D-Amino Acid Oxidase enzyme belongs to the FAD dependent oxidoreductase family, and acts on the CH-NH2 group of D-amino acid donors with oxygen as acceptor. It regulates the level of the neuromodulator D-serine in the brain. It has high activity towards D-DOPA and contributes to dopamine synthesis. It could act as a detoxifying agent which removes D-amino acids accumulated during aging. D-Amino Acid Oxidase acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. But does not act on acidic amino acids.

Gene Name: DAO

ID gène: 397134

UniProt: P00371