PDGFRA anticorps (C-Term)

N° du produit ABIN5611421

L’anticorps anti-PDGFRA Polyclonal Lapin est utilisé pour la détection de PDGFRA dans des échantillons de Humain, Souris et Rat. Il a été validé pour IHC (p) et EIA.

Aperçu rapide pour PDGFRA anticorps (C-Term) (ABIN5611421)

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PDGFRA est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-PDGFRA anticorps

Épitope: C-Term

Specificité: Specific for PDGFR, alpha and does not react with PDGFR, beta. Cellular Localization Cell membrane.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Protein A Chromatography.

Immunogène: A synthetic peptide derived from the C-terminus of the precursor form of Human PDGFR alpha.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Immunohistochemistry on Paraffin Sections: Use Ab at 5-10 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Positive Control: Ovariancarcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 0.2 % BSA as stabilizer, before the addition of Glycerol to 40 %.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur PDGFRA

Antigène: PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))

Autre désignation: CD140a / PDGFRA

Sujet: PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha and beta coded by two different genes. They possess five immunoglobulin like domains that are involved in ligand binding. Two tyrosine kinase domains are separated by a kinase insert to which PI-3 kinase can bind. PDGF AA can bind autophosphorylate and activate only PDGFR, alpha while PDGF BB can bind and activate to both PDGFR, alpha and beta.Synonyms: Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, PDGF Receptor alpha, PDGF-R-alpha

ID gène: 5156

NCBI Accession: NP_006197

UniProt: P16234

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling