Recombinant EPH Receptor A2 anticorps (AA 183-510)

N° du produit ABIN5668239

L’anticorps Human Monoclonal anti-EPH Receptor A2 a été validé pour WB, ELISA, FACS, IF, Agon et INA. Il convient pour détecter EPH Receptor A2 dans des échantillons de Humain.

Aperçu rapide pour Recombinant EPH Receptor A2 anticorps (AA 183-510) (ABIN5668239)

Antigène: EPH Receptor A2 (EPHA2)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Human

Clonalité: Monoclonal

Conjugué: Cet anticorp EPH Receptor A2 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Agonist (Agon), Internalization Assay (INA)

Clone: 1C1

Détail du produit anti-EPH Receptor A2 anticorps

Épitope: AA 183-510

Fonction: Anti-Human Ephrin Type A receptor 2 [1C1], Human IgG1, kappa

Specificité: 1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM, Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2.

Réactivité croisée: Souris

Attributs du produit:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: 1C1 was isolated after screening of a non-immunized human Fab phage library. Selection was based on the ability to bind soluble human recombinant EphA2 (residues 183-510). The Fab was subsequently converted to full length human IgG1.

Isotype: IgG1 kappa

Information d'application

Indications d'application: This antibody binds to human Ephrin type-A receptor 2, a receptor tyrosine kinase that binds to membrane-bound ephrin-A ligands residing on adjacent cells, triggering contact-dependent signaling into neighboring cells. Eph2A is involved in pattern formation during development, angiogenesis and osteoblastogenesis. Eph2A has also been found to be expressed in a variety of cancers. This antibody acts as an agonist of Eph2A signaling. The agonist properties of 1C1 is due to ligand mimicry by VDR3. After cell binding, the antibody is able to induce rapid tyrosine phosphorylation, internalization, and degradation of the EphA2 receptor.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur EPH Receptor A2

Antigène: EPH Receptor A2 (EPHA2)

Autre désignation: Ephrin Type A receptor 2

Sujet: EphA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK

UniProt: P29317

Pathways: Signalisation RTK