FASL anticorps (Biotin)

N° du produit ABIN5687581

L’anticorps Chèvre Polyclonal anti-FASL a été validé pour WB et ELISA. Il convient pour détecter FASL dans des échantillons de Humain.

Aperçu rapide pour FASL anticorps (Biotin) (ABIN5687581)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp FASL est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA

Détail du produit anti-FASL anticorps (Biotin)

Purification: Anti-hFasL/Apo1 specific antibody was purified by affinity chromatography and then biotinylated.

Immunogène: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL/Apo1 (human FasL/Apo1).

Information d'application

Indications d'application: ELISA:

To detect hFasL/Apo1 by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hFasL/Apo1.

Sandwich: To detect hFasL/Apo1L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FasL/Apo1L (XP-5130) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFasL/Apo1L.

Western Blot:

To detect hFasL/Apo1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL/Apo1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: -20 °C

Stockage commentaire: Fas Ligand antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Détails sur FASL

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: Fas Ligand

Sujet: Fas Ligand is an important member of the tumour necrosis factor (TNF) superfamily involved in membrane mediated apoptosis. Ligation of Fas by Fas Ligand or an anti Fas cross linking antibody, triggers activation of the caspase cascade. Functional impairment of the Fas / Fas Ligand system is associated with the development and progression of malignancies. Fas gene mutations have been suggested to have a role in testicular germ cell tumors. Tumor cells frequently exhibit de novo expression of Fas Ligand, which plays a significant role in local tissue destruction, metastatic spread, and immune escape of the tumor cells. The apoptosis of lymphocytes, which occurs in autoimmune diseases, is usually induced by the Fas/Fas Ligand system.

ID gène: 356

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity