GZMB anticorps (AA 7-227)

N° du produit ABIN5688512

Cet anticorps anti-GZMB est un anticorps Souris Monoclonal détectant GZMB dans WB, IHC, FACS, IP et ICC. Adapté pour Humain.

Aperçu rapide pour GZMB anticorps (AA 7-227) (ABIN5688512)

Antigène: GZMB (Granzyme B (GZMB))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GZMB est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: B18-1

Détail du produit anti-GZMB anticorps

Épitope: AA 7-227

Purification: Granzyme B (monoclonal B18.1) antibody was purified to ≥,95% in SDS-PAGE.

Immunogène: Granzyme B (monoclonal B18.1) antibody was raised against recombinant human granzyme B, amino acids 7-227.

Isotype: IgG2a

Information d'application

Indications d'application: Granzyme B antibody can be used for detection of human granzyme B by flow cytometry, immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: Permeabilized human T cells (5 x 105) were incubated on ice for 30 min in 50 μ,l FACS buffer (PBS, 5 % Fetal calf serum, 0.02 % azide) containing 1 μ,g of FITC- labeled B18.1 antibody. After washing in FACS buffer, cells were analyzed by flow cytometry. Method: Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1 % BSA, slides were incubated for 1 hr at room temperature (RT) with 20 μ,g/mL of B18.4 antibody in PBS, 0.1 % BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope. Method: : Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1 μ,g/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Granzyme B antibody can be stored at 4°C, stable for one year.

Détails sur GZMB

Antigène: GZMB (Granzyme B (GZMB))

Autre désignation: Granzyme B

ID gène: 3002

UniProt: P10144

Pathways: Apoptose, Caspase Cascade in Apoptosis