TRAIL anticorps

N° du produit ABIN5688718

L’anticorps anti-TRAIL Monoclonal Souris est utilisé pour la détection de TRAIL dans des échantillons de Humain. Il a été validé pour WB, ELISA et Neut.

Aperçu rapide pour TRAIL anticorps (ABIN5688718)

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TRAIL est non-conjugé

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Clone: 1-1_1A7-2B7

Détail du produit anti-TRAIL anticorps

Purification: This IgG1k antibody was purified from ascites fluid on a Protein A affinity column.

Immunogène: The monoclonal antibody was produced in Balb/c X ICR F1 mice using recombinant hTRAIL/APO-II as the immunizing antigen.

Information d'application

Indications d'application: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (10.0 ng/mL), a concentration of 0.30-0.60 μ,g/mL of this antibody is required.

ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 2.0-4.0 μ,g/mL of this antibody will detect at least 500 pg/mL of recombinant human TRAIL/APO-II when used with our biotinylated antigen affinity purified anti-human TRAIL/APO-II (XP-5289Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μ,g/mL.

Western Blot:

To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Stock: 4 °C,-20 °C

Stockage commentaire: Trail/Apo2L lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Détails sur TRAIL

Antigène: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Autre désignation: TRAIL

ID gène: 8743

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity