Caspase 8 anticorps (AA 389-479)

N° du produit ABIN5693195

Cet anticorps anti-Caspase 8 est un anticorps Lapin Polyclonal détectant Caspase 8 dans WB et ELISA. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour Caspase 8 anticorps (AA 389-479) (ABIN5693195)

Antigène: Caspase 8 (CASP8)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Caspase 8 est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Caspase 8 anticorps

Épitope: AA 389-479

Fonction: Anti-Caspase 8/CASP8 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Caspase 8/CASP8 Antibody Picoband® (ABIN5693195). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogène: E. coli-derived human Caspase 8 recombinant protein (Position: Q389-D479).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Activated neutrophils induce epithelial cell apoptosis through oxidant-dependent tyrosine dephosphorylation of caspase-8.Jia SH, et al. Am J Pathol, 2014 Apr. 2. Association between CASP8 -652 6N del polymorphism (rs3834129) and colorectal cancer risk: results from a multi-centric study.Pardini B, et al. PLoS One, 2014. 3. Intra- and interdimeric caspase-8 self-cleavage controls strength and timing of CD95-induced apoptosis.Kallenberger SM, et al. Sci Signal, 2014 Mar 11.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Caspase 8 (ABIN5693195)

Zhan, Hu, Yi, An, Huang: "[Corrigendum] Inhibitory activity of apogossypol in human prostate cancer in vitro and in vivo." dans: Molecular medicine reports, Vol. 17, Issue 6, pp. 8010, (2018) (PubMed).

Zhou, He, Wang, Xie, Liu, Liu, Song, Ma: "Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir." dans: International journal of molecular sciences, Vol. 17, Issue 6, (2017) (PubMed).

Zhang, Huang, Wang, Luo, Wang: "2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 3442-8, (2016) (PubMed).

Li, Wu, Sun, Zhao, Liu, Zhang: "Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells" dans: Asian Pacific journal of cancer prevention : APJCP, Vol. 17, Issue 11, pp. 4893-4898, (2016) (PubMed).

Lian, Ni, Dai, Su, Smith, Xu, He: "Sorafenib sensitizes (-)-gossypol-induced growth suppression in androgen-independent prostate cancer cells via Mcl-1 inhibition and Bak activation." dans: Molecular cancer therapeutics, Vol. 11, Issue 2, pp. 416-26, (2012) (PubMed).

Détails sur Caspase 8

Antigène: Caspase 8 (CASP8)

Autre désignation: CASP8

Sujet:

Synonyms: Caspase-8, CASP-8, Apoptotic cysteine protease, Apoptotic protease Mch-5, CAP4, FADD-homologous ICE/ced-3-like protease, FADD-like ICE, FLICE, ICE-like apoptotic protease 5, MORT1-associated ced-3 homolog, MACH, Caspase-8 subunit p18, Caspase-8 subunit p10, CASP8, MCH5

Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

Background: CASP8 is also known as CAP4, MACH or MCH5. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. In addtion, this protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.

Poids moléculaire: 55-60 kDa

ID gène: 841

UniProt: Q14790

Pathways: Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades