NUDT16 anticorps

N° du produit ABIN5705803

Détail du produit anti-NUDT16 anticorps

Antigène: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NUDT16 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Immunogène: nudix (nucleoside diphosphate linked moiety X)-type motif 16

Isotype: IgG

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

HeLa cells were subjected to SDS PAGE followed by western blot with FNab05901(NUDT16 antibody) at dilution of 1:2500

Restrictions: For Research Use only

Stockage

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze / thaw cycles.

Stock: -20 °C

Date de péremption: 12 months

Détails sur NUDT16

Antigène: NUDT16 (Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 16 (NUDT16))

Autre désignation: NUDT16 (NUDT16 Produits)

Synonymes: anticorps RGD1311387, anticorps 2310041H06Rik, anticorps 2810047L04Rik, anticorps 2900006H04Rik, anticorps AI851783, anticorps x29, anticorps nudix hydrolase 16, anticorps nudix (nucleoside diphosphate linked moiety X)-type motif 16, anticorps nudix hydrolase 16 L homeolog, anticorps Nudt16, anticorps NUDT16, anticorps nudt16.L

Sujet: Synonyms:Nudix motif 16, NUDT16, U8 snoRNA binding protein H29K, U8 snoRNA decapping enzyme Background:RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate(m7GpppG). Also hydrolysis m7G-and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA, metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation. Acts also as a phosphatase, hydrolyzes the non-canonical purine nucleotides inosine diphosphate(IDP) and deoxyinosine diphosphate(dITP) as well as guanosine diphosphate(GDP), deoxyguanosine diphosphate(dGDP), xanthine diphosphate(XDP), inosine triphosphate(ITP) and deoxyinosine triphosphate(ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy-and ribose forms(PubMed:20385596, PubMed:26121039). The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP(PubMed:20385596). Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions(PubMed:20385596).

Poids moléculaire: 21 kDa

ID gène: 131870

UniProt: Q96DE0

Pathways: Positive Regulation of Response to DNA Damage Stimulus