Histone H4 anticorps (2meLys20)
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- Antigène Voir toutes Histone H4 Anticorps
- Histone H4
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Épitope
- 2meLys20
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Reactivité
- Humain, Souris, Rat, Poulet, Xenopus laevis, Drosophila melanogaster, C. elegans, Plantes
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp Histone H4 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)
- Attributs du produit
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Synonyms: rabbit anti-Histone H4 dimethyl Lys20 antibody, H4K20me2, HIST2H4B, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, histone cluster 4, H4, histone 4, H4
Background: When K20 is di-methylated on H4, the downstream effect is silencing of genes. This modification is also necessary and sufficient for 53BP1 binding, which is a prerequisite for DNA repair, a highly conserved mechanism. H4K20me2 is associated with hypoacetylation, and inactivation of certain genes. NSD1 and Suv420h1/2 trigger the dimethylation of K20, but only after SET8 catalyzes the mono-methylation of the same lysine, indicating a complex regulation of this modification that can so seriously affect replication and stability of genomic information. Anti-Histone H4 are ideal for researchers interested in Chromatin Modifiers, Chromatin Research, DNA Repair, DNA replication Transcription Translation and Splicing, Histones and Modified Histones, and Epigenetics research.
Gene Name: HIST4H4
- Purification
- Anti-Histone H4 [Dimethyl Lys20] was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human Histone H4. A BLAST analysis was used to suggest cross-reactivity with Human, mouse, and C. elegans. Predicted to react with most mammal species. Cross-reactivity with Histone H4 from other sources has not been determined.
- Immunogène
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Immunogen: Histone H4 [Dimethyl Lys20] affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic dimethylated peptide surrounding Lysine 20 of human Histone H4.
Immunogen Type: Peptide
- Isotype
- IgG
- Top Product
- Discover our top product Histone H4 Anticorps primaire
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- Indications d'application
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Immunohistochemistry Dilution: 1:50
Application Note: Anti-Histone H4 [Dimethyl Lys20] antibody is useful for Western Blot, Dot Blot, Chromatin Immunoprecipitation, Immunofluorescence, and Immunocytochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~13 kDa corresponding to Histone H4 protein by Western Blotting in HeLa histone prep lysate or the appropriate cell lysate or extract. Epi-Plus™ antibody production in collaboration with Novus Biologicals.
ChIP Dilution: 2-5 μg/million cells
Western Blot Dilution: 1:2000
IF Microscopy Dilution: 1:50
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.53 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- Histone H4
- Abstract
- Histone H4 Produits
- Synonymes
- anticorps hist1h4l, anticorps H1ft, anticorps Hist4, anticorps Hist4h4, anticorps H4, anticorps X04652, anticorps FO108, anticorps H4/n, anticorps H4F2, anticorps H4FN, anticorps HIST2H4, anticorps H4C13, anticorps H4C7, anticorps F8N16.2, anticorps F8N16_2, anticorps histone H4, anticorps BcDNA:RH52884, anticorps CG3379, anticorps Dmel\CG3379, anticorps H4r, anticorps His4-88CD, anticorps His4R, anticorps histone cluster 1, H4a L homeolog, anticorps histone cluster 1 H4 family member B, anticorps histone cluster 2, H4, anticorps histone cluster 2 H4 family member a, anticorps histone H4, anticorps Histone H4 replacement, anticorps Histone H4, anticorps hist1h4a.L, anticorps Hist1h4b, anticorps Hist2h4, anticorps HIST2H4A, anticorps H4, anticorps H4C14, anticorps HIS4, anticorps HIST2H4, anticorps His4r, anticorps his-38
- ID gène
- 121504
- NCBI Accession
- NP_001029249
- UniProt
- P62805
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