F11R anticorps (C-Term, pTyr280)

N° du produit ABIN5706868

Détail du produit anti-F11R anticorps

Antigène: F11R (F11 Receptor (F11R))

Épitope: C-Term, pTyr280

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp F11R est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Attributs du produit:

Synonyms: rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA

Background: Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

Gene Name: F11R

Purification: Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.  A BLAST analysis was used to suggest reactivity with this protein from human and feline based on 100% homology for the immunogen sequence.

Immunogène:

Immunogen: Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1.0 μg/mL

ELISA Dilution: 5.0 μg/mL

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.95 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,  pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Références pour anticorps anti-F11R (ABIN5706868)

Tapia, Kralicek, Hecht: "Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells." dans: International journal of molecular sciences, Vol. 21, Issue 2, (2020) (PubMed).

Fan, Weight, Luissint, Hilgarth, Brazil, Ettel, Nusrat, Parkos: "Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation." dans: Molecular biology of the cell, Vol. 30, Issue 5, pp. 566-578, (2019) (PubMed).

Détails sur F11R

Antigène: F11R (F11 Receptor (F11R))

Autre désignation: JAM A (F11R Produits)

Synonymes: anticorps CD321, anticorps JAM, anticorps JAM1, anticorps JAMA, anticorps JCAM, anticorps KAT, anticorps PAM-1, anticorps 9130004G24, anticorps AA638916, anticorps ESTM33, anticorps JAM-1, anticorps JAM-A, anticorps Jcam, anticorps Jcam1, anticorps Ly106, anticorps Jam1, anticorps F11 receptor, anticorps F11R, anticorps F11r

ID gène: 50848

NCBI Accession: NP_058642

Pathways: Cell-Cell Junction Organization