AIM2 anticorps (C-Term)

N° du produit ABIN571131

Cet anticorps anti-AIM2 est un anticorps Chèvre Polyclonal détectant AIM2 dans WB, ELISA, IHC, IF et FACS. Adapté pour Humain.

Aperçu rapide pour AIM2 anticorps (C-Term) (ABIN571131)

Antigène: AIM2 (Absent in Melanoma 2 (AIM2))

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIM2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-AIM2 anticorps

Épitope: C-Term

Fonction: AIM2

Séquence: GEKLQLTSGV HSTIK

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-GEKLQLTSGVHSTIK, from the C Terminus of the protein sequence according to NP_004824.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx 37 kDa band observed in lysates of cell line HeLa (calculated MW of 39.0 kDa according to Human NP_004824.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:8000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur AIM2

Antigène: AIM2 (Absent in Melanoma 2 (AIM2))

Autre désignation: AIM2

Sujet: AIM2, absent in melanoma 2, PYHIN4

Poids moléculaire: 39.0kDa

ID gène: 9447

NCBI Accession: NP_004824

Pathways: Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome