CTNNB1 anticorps (AA 36-44)

N° du produit ABIN600589

Cet anticorps Souris Monoclonal détecte spécifiquement CTNNB1 dans WB, IHC, ELISA et ICC. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour CTNNB1 anticorps (AA 36-44) (ABIN600589)

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CTNNB1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Détail du produit anti-CTNNB1 anticorps

Épitope: AA 36-44

Specificité: Specific for the active form of beta-catenin, non-phosphorylated on Ser37 and Thr41.1 A non-specific protein, Mr 130kD, was also detected when used at higher concentrations. Species cross-reactivity: Mouse and rat (100 % ). Predicted to cross-react with frog and chicken (97/100), porcine (99/100) and zebrafish (91/100) based on epitope sequence homology. Does not cross-react with Drosophila. Others not tested.

Purification: Protein G purified

Immunogène: Fusion protein corresponding to residues 1-100 of human beta-catenin with a C-terminal histidine tag. Epitope: Corresponds to aa 36-44 (HSGATTTAP) of human beta-catenin, Mr 92kD.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: ELISA, ICC, IHC, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects beta-catenin in RIPA lysates from A431 cells. Immunohistochemistry: Detects b-catenin in mouse embryo sections. Immunocytochemistry: Detects b-catenin in LiCl-treated 293Tcells fixed with methanol.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4 No stabilizing proteins added. No preservative added. Also available w/ azide. See C2069-47

Agent conservateur: Sodium azide, Without preservative

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing

Stock: 4 °C,-20 °C

Stockage commentaire: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 1 year at -20°C.

Détails sur CTNNB1

Antigène: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Autre désignation: CTNNB1 / Beta Catenin

Sujet: Name/Gene ID: CTNNB1
Family: Catenin

Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin

ID gène: 1499

UniProt: P35222

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2