Villin 1 anticorps (AA 179-311) (Biotin)

N° du produit ABIN6186943

Détail du produit anti-Villin 1 anticorps (Biotin)

Antigène: Villin 1 (VIL1)

Épitope: AA 179-311

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Villin 1 est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: Mouse Monoclonal anti-Villin (VIL1/1314), Biotin Conjugate

Attributs du produit: This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogène: Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)

Clone: VIL1-1314

Isotype: IgG1 kappa

Information d'application

Indications d'application: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.25-0.5 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 1-2 μg/mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Commentaires:

A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Détails sur Villin 1

Antigène: Villin 1 (VIL1)

Autre désignation: Villin (VIL1 Produits)

Synonymes: anticorps D2S1471, anticorps VIL, anticorps Vil, anticorps vil1, anticorps MGC64339, anticorps VIL1, anticorps vil1l, anticorps zgc:55779, anticorps villin 1, anticorps villin 1 S homeolog, anticorps VIL1, anticorps Vil1, anticorps vil1.S, anticorps vil1

Sujet: VIL1, Villin-1, Villin1

Poids moléculaire: 93 kDa

ID gène: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization