MFGE8 anticorps

N° du produit ABIN6252078

Cet anticorps Souris Monoclonal détecte spécifiquement MFGE8 dans WB, FACS, IF et IHC (p). Il présente une réactivité envers Humain.

Aperçu rapide pour MFGE8 anticorps (ABIN6252078)

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MFGE8 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM291

Détail du produit anti-MFGE8 anticorps

Purification: Protein G affinity chromatography

Immunogène: A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the MFG-E8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur MFGE8

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Autre désignation: MFG-E8

Sujet: Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

ID gène: 4240

UniProt: Q08431

Pathways: SARS-CoV-2 Protein Interactome