CD3 epsilon anticorps (Cytoplasmic Domain)

N° du produit ABIN6252415

L’anticorps anti-CD3 epsilon Monoclonal Souris est utilisé pour la détection de CD3 epsilon dans des échantillons de Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin et Cobaye. Il a été validé pour FACS, WB, IF et IHC (p).

Aperçu rapide pour CD3 epsilon anticorps (Cytoplasmic Domain) (ABIN6252415)

Antigène: CD3 epsilon (CD3E)

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Cheval, Lapin, Cobaye

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD3 epsilon est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PC3-188A

Détail du produit anti-CD3 epsilon anticorps

Épitope: AA 156-168, Cytoplasmic Domain

Purification: Protein G affinity chromatography

Immunogène: Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG1

Information d'application

Indications d'application: WB: 1-2 μg/mL

IHC (FFPE): 1-2 μg/mL for 30 min at RT

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Optimal dilution of the CD3e antibody should be determined by the researcher.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur CD3 epsilon

Antigène: CD3 epsilon (CD3E)

Autre désignation: CD3e

Sujet: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

ID gène: 916

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway