IRAK4 anticorps (pSer346, pThr345)

N° du produit ABIN6256548

L’anticorps Lapin Polyclonal anti-IRAK4 a été validé pour WB, ELISA et IHC. Il convient pour détecter IRAK4 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour IRAK4 anticorps (pSer346, pThr345) (ABIN6256548)

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IRAK4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Détail du produit anti-IRAK4 anticorps

Épitope: pSer346, pThr345

Specificité: Phospho-IRAK4 (Thr345/Ser346) Antibody detects endogenous levels of IRAK4 only when phosphorylated at Thr345/Ser346.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human IRAK4 around the phosphorylation site of Thr345/Ser346.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur IRAK4

Antigène: IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))

Autre désignation: IRAK4

Sujet:

Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Gene: IRAK4

Poids moléculaire: 60 kDa

ID gène: 51135

UniProt: Q9NWZ3

Pathways: Signalisation NF-kappaB, Signalisation TLR, Activation of Innate immune Response, Toll-Like Receptors Cascades