GNAI3 anticorps (N-Term)

N° du produit ABIN6258933

Détail du produit anti-GNAI3 anticorps

Antigène: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Épitope: N-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GNAI3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: GNAI3 Antibody detects endogenous levels of total GNAI3.

Homologie: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human GNAI3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Références pour anticorps anti-GNAI3 (ABIN6258933)

Costas-Insua, Moreno, Maroto, Ruiz-Calvo, Bajo-Grañeras, Martín-Gutiérrez, Diez-Alarcia, Vilaró, Cortés, García-Font, Martín, Espina, Botta, Ginés, McCormick, Sánchez-Prieto, Galve-Roperh, Mengod et al.: "Identification of BiP as a CB1 Receptor-Interacting Protein That Fine-Tunes Cannabinoid Signaling in the Mouse Brain. ..." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 41, Issue 38, pp. 7924-7941, (2021) (PubMed).

Détails sur GNAI3

Antigène: GNAI3 (Guanine Nucleotide Binding Protein (G Protein), alpha Inhibiting Activity Polypeptide 3 (GNAI3))

Autre désignation: GNAI3 (GNAI3 Produits)

Synonymes: anticorps AI158965, anticorps AW537698, anticorps Galphai3, anticorps Gnai-3, anticorps 87U6, anticorps ARCND1, anticorps GIP3A, anticorps G protein subunit alpha i3, anticorps guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3, anticorps guanine nucleotide binding protein (G protein), alpha inhibiting 3, anticorps G protein subunit alpha i3 S homeolog, anticorps GNAI3, anticorps gnai3, anticorps Gnai3, anticorps gnai3.S

Sujet:

Description: Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:8774883, PubMed:18434541, PubMed:19478087). Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels (PubMed:19478087). Stimulates the activity of receptor-regulated K+ channels (PubMed:2535845). The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division (PubMed:17635935).

Gene: GNAI3

Poids moléculaire: 41 kDa

ID gène: 2773

UniProt: P08754

Pathways: cAMP Metabolic Process, G-protein mediated Events