PRKAG1/2/3 anticorps

N° du produit ABIN6259312

Détail du produit anti-PRKAG1/2/3 anticorps

Antigène: PRKAG1/2/3 (Protein Kinase, AMP-Activated, gamma 1/2/3 Non-Catalytic Subunit (PRKAG1/2/3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKAG1/2/3 est non-conjugé

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificité: PRKAG1/2/3 Antibody detects endogenous levels of total PRKAG1/2/3

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide

Isotype: IgG

Information d'application

Indications d'application: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Détails sur PRKAG1/2/3

Antigène: PRKAG1/2/3 (Protein Kinase, AMP-Activated, gamma 1/2/3 Non-Catalytic Subunit (PRKAG1/2/3))

Autre désignation: PRKAG1/2/3 (PRKAG1/2/3 Produits)

Sujet:

Description: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene: PRKAG1

Poids moléculaire: 38 kDa

ID gène: 5571

UniProt: P54619, Q9UGJ0, Q9UGI9