EIF4A3 anticorps (Internal Region)

N° du produit ABIN6261488

Détail du produit anti-EIF4A3 anticorps

Antigène: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp EIF4A3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: EIF4A3 Antibody detects endogenous levels of total eIF4A3.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human eIF4A3, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur EIF4A3

Antigène: EIF4A3 (Eukaryotic Translation Initiation Factor 4A3 (EIF4A3))

Autre désignation: EIF4A3 (EIF4A3 Produits)

Synonymes: anticorps DDX48, anticorps NMP265, anticorps NUK34, anticorps eIF4AIII, anticorps 2400003O03Rik, anticorps Ddx48, anticorps eIF4A-III, anticorps mKIAA0111, anticorps ddx48, anticorps nuk34, anticorps nmp265, anticorps eif4aiii, anticorps EIF4A3, anticorps eif4a3, anticorps zgc:56139, anticorps XeIF-4AIII, anticorps eif4a3-B, anticorps eukaryotic translation initiation factor 4A3, anticorps eukaryotic initiation factor 4A-III, anticorps eukaryotic translation initiation factor 4A3 S homeolog, anticorps Eukaryotic initiation factor 4A-III, anticorps EIF4A3, anticorps Eif4a3, anticorps eif4a3, anticorps LOC100185906, anticorps eif4a3.S, anticorps if4a3

Sujet:

Description: ATP-dependent RNA helicase (PubMed:16170325). Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:22961380, PubMed:28502770, PubMed:28076346). Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly (PubMed:22203037). Involved in craniofacial development (PubMed:24360810).

Gene: EIF4A3

Poids moléculaire: 47 kDa

ID gène: 9775

UniProt: P38919