Raptor anticorps (C-Term)

N° du produit ABIN6264641

Cet anticorps Lapin Polyclonal détecte spécifiquement Raptor dans ELISA, WB, IHC, IF et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Raptor anticorps (C-Term) (ABIN6264641)

Antigène: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Raptor est non-conjugé

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-Raptor anticorps

Épitope: C-Term

Specificité: Raptor Antibody detects endogenous levels of total Raptor.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human Raptor, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur Raptor

Antigène: Raptor (RPTOR) (Regulatory Associated Protein of mTOR, Complex 1 (RPTOR))

Autre désignation: RPTOR

Sujet:

Description: Involved in the control of the mammalian target of rapamycin complex 1 (mTORC1) activity which regulates cell growth and survival, and autophagy in response to nutrient and hormonal signals, functions as a scaffold for recruiting mTORC1 substrates. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Involved in ciliogenesis.

Gene: RPTOR

Poids moléculaire: 149kDa

ID gène: 57521

UniProt: Q8N122

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Autophagy, BCR Signaling, L'effet Warburg