Bcl-2 anticorps (AA 41-54)

N° du produit ABIN6294520

Cet anticorps Souris Monoclonal détecte spécifiquement Bcl-2 dans WB, IHC, IF, FACS, ELISA et Coat. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Bcl-2 anticorps (AA 41-54) (ABIN6294520)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bcl-2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Coating (Coat)

Détail du produit anti-Bcl-2 anticorps

Épitope: AA 41-54

Fonction: Mouse anti-Human Bcl-2 Antibody [Sodium Azide Free]

Specificité: Cytoplasmic, membrane

Purification: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Immunogène: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein was used as the immunogen for this antibody.

Information d'application

Indications d'application: ELISA: order BSA-free format for coating
FACS: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: Apoptosis regulator Bcl-2

Sujet:

Target Description: This antibody recognizes a protein of 25-26 kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of Bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 protein and the small number in which the neoplastic cells are Bcl-2 negative.

Gene Symbol: BCL2

ID gène: 596

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling