Bcl-2 anticorps (AA 41-54)

N° du produit ABIN6294524

L’anticorps anti-Bcl-2 Monoclonal Souris est utilisé pour la détection de Bcl-2 dans des échantillons de Humain. Il a été validé pour WB, IHC, IF, FACS et IP.

Aperçu rapide pour Bcl-2 anticorps (AA 41-54) (ABIN6294524)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bcl-2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Détail du produit anti-Bcl-2 anticorps

Épitope: AA 41-54

Fonction: Mouse anti-Human Bcl2 Antibody Cocktail [Sodium Azide Free]

Specificité: Cytoplasmic, membrane

Purification: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.

Immunogène: A synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG) of the human protein was used as the immunogen for both antibodies in this Bcl2 antibody cocktail.

Information d'application

Indications d'application: Flow: 0.5-1 μg/million cells
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunoprecipitation: 0.5-1 μg/500 μg protein lysate
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: Apoptosis regulator Bcl-2

Sujet:

Target Description: B-cell lymphoma 2 is an oncogene that regulates apoptosis, having both inducing and inhibiting functions. In cancer cells, Bcl2 binds pro-apoptotic proteins, preventing programmed cell death from occurring. Overexpression is seen in many cancers including lung, breast, prostate and lymphomas. In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies.

Gene Symbol: BCL2

ID gène: 596

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling