Bcl-2 anticorps (AA 41-54) (PE)

N° du produit ABIN638444

L’anticorps Souris Monoclonal anti-Bcl-2 a été validé pour ICFC. Il convient pour détecter Bcl-2 dans des échantillons de Humain. Il y a 5+ publications disponibles.

Aperçu rapide pour Bcl-2 anticorps (AA 41-54) (PE) (ABIN638444)

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Bcl-2 est conjugé à/à la PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: Bcl-2-100

Détail du produit anti-Bcl-2 anticorps (PE)

Épitope: AA 41-54

Fonction: Anti-Hu BCL2 PE

Specificité: The mouse monoclonal antibody Bcl-2/100 recognizes Bcl2, a 26 kDa intracellular protooncogen with anti-apoptotic effect, expressed in outer mitochondrial membrane, endoplasmic reticulum and nuclear envelope.

Aucune reactivité croisée: Souris

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Synthetic peptide corresponding to the amino acids 41-54 of human Bcl2

Isotype: IgG1

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Stockage

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.
Avoid prolonged exposure to light.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-Bcl-2 (PE) (ABIN638444)

Joubert, Marais, Maritz: "Influence of 2-methoxyestradiol on MCF-7 cells: an improved differential interference contrasting technique and Bcl-2 and Bax protein expression levels." dans: Biocell : official journal of the Sociedades Latinoamericanas de Microscopi?a Electronica ... et. al, Vol. 33, Issue 1, pp. 67-70, (2009) (PubMed).

Laforge, Petit, Estaquier, Senik: "Commitment to apoptosis in CD4(+) T lymphocytes productively infected with human immunodeficiency virus type 1 is initiated by lysosomal membrane permeabilization, itself induced by the isolated expression of the viral protein Nef." dans: Journal of virology, Vol. 81, Issue 20, pp. 11426-40, (2007) (PubMed).

Gugasyan, Christou, OReilly, Strasser, Gerondakis: "Bcl-2 transgene expression fails to prevent fatal hepatocyte apoptosis induced by endogenous TNFalpha in mice lacking RelA." dans: Cell death and differentiation, Vol. 13, Issue 7, pp. 1235-7, (2006) (PubMed).

Laflamme, Israël-Assayag, Cormier: "Apoptosis of bronchoalveolar lavage lymphocytes in hypersensitivity pneumonitis." dans: The European respiratory journal, Vol. 21, Issue 2, pp. 225-31, (2003) (PubMed).

Soilu-Hänninen, Ekert, Bucci, Syroid, Bartlett, Kilpatrick: "Nerve growth factor signaling through p75 induces apoptosis in Schwann cells via a Bcl-2-independent pathway." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 12, pp. 4828-38, (1999) (PubMed).

Détails sur Bcl-2

Antigène: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Autre désignation: BCL2

Sujet: BCL2 apoptosis regulator,Bcl2 (B cell chronic lymphatic leukemia protein 2) is a proto-oncogen, which can contribute to tumorigenesis by counteracting apoptosis in various cell types. The anti-apoptotic effect of Bcl2 is performed by its interactions with suppressors and agonists of cell death and under physiological conditions it is regulated by proteolytic processing and phosphorylation. Bcl2 expression can be detected mainly in lymphoid tissues and in the basal cells of epithelial tissues. It is also a marker that can help in classification of lymphoproliferative diseases and in prognostics of some epithelial neoplasms.,Bcl-2, PPP1R50

ID gène: 596

UniProt: P10415

Pathways: Signalisation MAPK, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling