HDAC2 anticorps (Internal Region)

N° du produit ABIN6391348

Cet anticorps Chèvre Polyclonal détecte spécifiquement HDAC2 dans WB, ELISA, IF et FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour HDAC2 anticorps (Internal Region) (ABIN6391348)

Antigène: HDAC2 (Histone Deacetylase 2 (HDAC2))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-HDAC2 anticorps

Épitope: Internal Region

Fonction: Hdac2 (mouse)

Séquence: PEDAVHEDSG DE

Specificité: This antibody imay cross-react with HDAC1

Réactivité croisée: Boeuf (Vache), Chien, Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 55-60 kDa band observed in nuclear lysates of cell line HEK293 (calculated MW of 55.3 kDa according to NP_032255.2). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells and additionally in the membrane and cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur HDAC2

Antigène: HDAC2 (Histone Deacetylase 2 (HDAC2))

Autre désignation: Hdac2

Sujet: Hdac2, histone deacetylase 2, D10Wsu179e, YAF1, Yy1bp, mRPD3, HD2, OTTMUSP00000022803, YY1 transcription factor-binding protein

ID gène: 3066, 15182, 84577

NCBI Accession: NP_032255

Pathways: Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2