H2AFX anticorps (pSer139)

N° du produit ABIN6654164

Cet anticorps anti-H2AFX est un anticorps Lapin Monoclonal détectant H2AFX dans WB, IF, ICC et IHC (p). Adapté pour Humain et Souris.

Aperçu rapide pour H2AFX anticorps (pSer139) (ABIN6654164)

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp H2AFX est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AbH41

Détail du produit anti-H2AFX anticorps

Épitope: pSer139

Purification: Protein A affinity

Immunogène: The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,IF/ICC: 2-4 μg/mL

Restrictions: For Research Use only

Stockage

Buffer: Antibody in PBS with 0.02 % sodium azide and 50 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Détails sur H2AFX

Antigène: H2AFX (H2A Histone Family, Member X (H2AFX))

Autre désignation: Histone H2AX

Sujet: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]

UniProt: P16104

Pathways: Telomere Maintenance, Réparation de l'ADN, Positive Regulation of Response to DNA Damage Stimulus