BAX anticorps (Internal Region)

N° du produit ABIN6655295

Détail du produit anti-BAX anticorps

Antigène: BAX (BCL2-Associated X Protein (BAX))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp BAX est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification: This product was purified from concentrated tissue culture supernate by Protein A/G chromatography. This antibody is specific for human BAX protein. Cross-reactivity with BAX protein from other sources has not been determined.

Immunogène:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human BAX protein. The hybridoma was produced by fusing New Zealand White rabbit splenocytes and myeloma cells using conventional technology.

Immunogen Type: Peptide

Clone: SP47

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50

Application Note: This monoclonal antibody is suitable for ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 21 kDa in size corresponding to BAX protein by western blotting in the appropriate cell lysate or extract. BAX is found in normal breast tissue as a cytoplasmic protein and also at the cell membrane. For immunohistochemistry, samples should be formalin fixed and paraffin embedded. Deparaffinize slides using xylene or xylene alternatives and graded alcohols. Staining requires boiling of sections in 10 mM citrate buffer pH 6.0 for 10 min followed by cooling at RT for 20 min.

ELISA Dilution: 1:20,000

Flow Cytometry Dilution: 1:100

Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.1 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze. The user must validate any other storage conditions. When properly stored, the reagent is stable to the date indicated on the label. Do not use the reagent beyond the expiration date. Contains 1% BSA.

Détails sur BAX

Antigène: BAX (BCL2-Associated X Protein (BAX))

Autre désignation: BAX (BAX Produits)

Synonymes: anticorps BAX-ALPHA, anticorps bax-A, anticorps xBax, anticorps xlbax, anticorps BAX, anticorps bax, anticorps fj16e01, anticorps wu:fc50b10, anticorps wu:fj16e01, anticorps BCL2L4, anticorps zgc:112983, anticorps BCL2 associated X, apoptosis regulator, anticorps BCL2-associated X protein L homeolog, anticorps BCL2-associated X protein, anticorps bcl2-associated X protein, a, anticorps bcl2-associated X protein, b, anticorps BAX, anticorps bax.L, anticorps bax, anticorps baxa, anticorps Bax, anticorps baxb

Sujet:

Synonyms: BAX Bcl-2-like protein 4 BCL2L4

Background: BAX, also known as Bcl-2-like protein 4 and BCL2L4 is known to accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. BAX induces the release of cytochrome c, activation of CASP3, and thereby apoptosis. BAX protein is expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histyocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines. Isoform alpha is considered to be the 'canonical' sequence.

Gene Name: BAX

ID gène: 581

UniProt: Q07812

Pathways: Signalisation p53, Signalisation PI3K-Akt, Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response