CD11b anticorps (APC)

N° du produit ABIN6655309

N° du produit (Fournisseur): 200-326-n20

L’anticorps anti-CD11b Monoclonal Souris est utilisé pour la détection de CD11b dans des échantillons de Humain. Il a été validé pour FACS, IP et FM.

Aperçu rapide pour CD11b anticorps (APC) (ABIN6655309)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la APC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: ICRF44

Détail du produit anti-CD11b anticorps (APC)

N° du produit (Fournisseur): 200-326-n20

Fournisseur: Rockland

Fonction: CD11b Allophycocyanin Antibody

Réactivité croisée (Details): Reactivity is observed against human CD11b, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Swine.

Purification: Allophycocyanin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b.

Stérilité: Sterile filtered

Immunogène: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Commentaires:

Anti-CD11b is tested for Flow Cytometry and useful in Immunoprecipitation and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet:

Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A

Background: The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells.

Gene Name: ITGAM

ID gène: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation