DDIT4 anticorps (pThr23, pThr25)

N° du produit ABIN6655385

Détail du produit anti-DDIT4 anticorps

Antigène: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Épitope: pThr23, pThr25

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDIT4 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: Anti-REDD1 pT23/pT25 Antibody is directed against REDD1 protein phosphorylated at T23 and T25. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated REDD1. Reactivity against REDD1 occurs from human sources. However, reactivity is also expected against non-human primate based on 100% sequence homology.

Immunogène:

Immunogen: Anti-Phospho-Thr23/25 REDD1 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Thr23/25 of human REDD1 conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Phospho-Thr23/25 REDD1 Antibody is suitable for Western Blots and is specific for the ~34 kDa REDD1 phosphorylated at Thr23/25. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:5,000

Western Blot Dilution: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Agent conservateur: Without preservative

Stock: RT,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Détails sur DDIT4

Antigène: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Autre désignation: REDD1 (DDIT4 Produits)

Synonymes: anticorps redd1, anticorps Dig2, anticorps REDD-1, anticorps REDD1, anticorps 5830413E08Rik, anticorps AA415483, anticorps Rtp801, anticorps dig2, anticorps rtp801, anticorps sb:cb821, anticorps zgc:64114, anticorps DNA damage inducible transcript 4, anticorps DNA-damage-inducible transcript 4, anticorps DNA damage inducible transcript 4 S homeolog, anticorps ddit4, anticorps DDIT4, anticorps Ddit4, anticorps ddit4.S

Sujet:

Synonyms: DNA damage-inducible transcript 4 protein, HIF-1 responsive protein RTP801, Protein regulated in development and DNA damage response 1

Background: REDD1, Regulated in Development and DNA damage responses 1, is induced by hypoxia, cell stress, and apoptosis. Reduced REDD1 levels can sensitize cells towards apoptosis, where elevated levels of REDD1 induced by hypoxia can desensitize cells to apoptotic stimuli. REDD1 has a crucial role in inhibiting mammalian rapamycin complex 1 (mTORC1) signaling during hypoxic stress. It has been shown that the rapid degradation of REDD1 is mediated by the CUL4A-DDB1-ROC1-b-TRCP E3 ligase complex and is regulated by REDD1 phosphorylation at Thr 25, Thr 23 and Ser 19 through the activity of GSK3b. REDD1 pT23/25 Antibody is ideal for researchers interested in cancer research.

Gene Name: DDIT4

ID gène: 54541

NCBI Accession: NP_061931

UniProt: Q9NX09

Pathways: Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process