MEG3 anticorps (pSer679, pSer683)

N° du produit ABIN6657452

Détail du produit anti-MEG3 anticorps

Antigène: MEG3 (FAM129B) (Family with Sequence Similarity 129, Member B (FAM129B))

Épitope: pSer679, pSer683

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MEG3 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Souris

Purification: Anti-Phospho-Ser679/683 FAM129B Antibody is directed against human FAM129B protein phosphorylated at S679 and S683. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated FAM129B. Minimal reactivity occurs against non-phosphorylated FAM129B. Reactivity against FAM129B occurs from mouse sources. However, reactivity is also expected against human and non-human primate based on 100% sequence homology.

Immunogène:

Immunogen: Anti-Phospho-Ser679/683 FAM129B Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser679/683 of human FAM129B conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Phospho-Ser679/683 FAM129B Antibody is suitable for Western Blots and is specific for the ~83 kDa FAM129B phosphorylated at Ser679/683. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to generate the antibody but not by the corresponding dephospho-peptide.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Agent conservateur: Without preservative

Stock: RT,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilut

Détails sur MEG3

Antigène: MEG3 (FAM129B) (Family with Sequence Similarity 129, Member B (FAM129B))

Autre désignation: FAM129B (FAM129B Produits)

Synonymes: anticorps C9orf88, anticorps MEG-3, anticorps MINERVA, anticorps OC58, anticorps bA356B19.6, anticorps MGC82535, anticorps 9130404D14Rik, anticorps RGD1307018, anticorps fam129b, anticorps zgc:158319, anticorps family with sequence similarity 129 member B, anticorps family with sequence similarity 129 member B S homeolog, anticorps family with sequence similarity 129, member B, anticorps family with sequence similarity 129, member Ba, anticorps FAM129B, anticorps fam129b.S, anticorps fam129b, anticorps Fam129b, anticorps fam129ba

Sujet:

Synonyms: Niban-like protein 1, Meg-3, Melanoma invasion by ERK, MINERVA, Protein FAM129B

Background: FAM129B, also known as Niban-like protein 1, belongs to a poorly characterized protein family with unknown category and function. Increased expression of the Niban gene has been observed in renal carcinomas. Suppression of FAM129B expression in HeLa cells has been seen to promote apoptosis, suggesting that it can modulate cell death signaling, and may be involved in the ER stress response. FAM129B is also up-regulated in various types of thyroid tumors and Hashimoto's thyroiditis. It has been suggested that the MAP kinase dependent phosphorylation of FAM129B is important in controlling melanoma cells, as inhibition of B/Raf/MKK/ERK in melanoma cells represses invasion. It is believed that phosphorylated FAM129B not only derepresses invasion, but also regulates events that promote invasion. FAM129B pS679/683 Antibody is ideal for researchers interested in cancer research.

Gene Name: FAM129B

ID gène: 64855

UniProt: Q96TA1