MCL-1 anticorps (Internal Region)

N° du produit ABIN6657787

Détail du produit anti-MCL-1 anticorps

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Épitope: Internal Region

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCL-1 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée (Details): Cross reactivity with Mcl-1 from other sources is unknown

Purification: Anti-Mcl-1 affinity purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence. .

Immunogène:

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Rabbit Anti-Mcl-1 antibody is suitable for western blotting, immunoprecipitation, and ELISA. This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size. The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Références pour anticorps anti-MCL-1 (ABIN6657787)

Dong, Vaux: "Glucocorticoids can induce BIM to trigger apoptosis in the absence of BAX and BAK1." dans: Cell death & disease, Vol. 11, Issue 6, pp. 442, (2021) (PubMed).

Schubert, Rapp, Brauns-Schubert, Schlicher, Stock, Wissler, Weiß, Charvet, Borner, Maurer: "Requirement of GSK-3 for PUMA induction upon loss of pro-survival PI3K signaling." dans: Cell death & disease, Vol. 9, Issue 5, pp. 470, (2018) (PubMed).

Guerra, Bird, Harvey, Dharia, Korshavn, Prew, Stegmaier, Walensky: "Precision Targeting of BFL-1/A1 and an ATM Co-dependency in Human Cancer." dans: Cell reports, Vol. 24, Issue 13, pp. 3393-3403.e5, (2018) (PubMed).

Girnius, Davis: "JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins." dans: Cell reports, Vol. 21, Issue 7, pp. 1910-1921, (2018) (PubMed).

Omari, Waters, Naranian, Kim, Perumalsamy, Chi, Greenblatt, Moley, Opferman, Jurisicova: "Mcl-1 is a key regulator of the ovarian reserve." dans: Cell death & disease, Vol. 6, pp. e1755, (2016) (PubMed).

Schuler, Baumgartner, Klepsch, Chamson, Müller-Holzner, Watson, Oh, Hennighausen, Tymoszuk, Doppler, Villunger: "The BH3-only protein BIM contributes to late-stage involution in the mouse mammary gland." dans: Cell death and differentiation, Vol. 23, Issue 1, pp. 41-51, (2016) (PubMed).

Doi, Liu, Gowda, Barth, Claxton, Amin, Loughran, Wang: "Maritoclax induces apoptosis in acute myeloid leukemia cells with elevated Mcl-1 expression." dans: Cancer biology & therapy, Vol. 15, Issue 8, pp. 1077-86, (2015) (PubMed).

Rutherford, Childs, Ohotski, McGlynn, Riddick, MacFarlane, Tasker, Pyne, Pyne, Edwards, Palmer: "Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1." dans: Cell death & disease, Vol. 4, pp. e927, (2014) (PubMed).

Perciavalle, Stewart, Koss, Lynch, Milasta, Bathina, Temirov, Cleland, Pelletier, Schuetz, Youle, Green, Opferman: "Anti-apoptotic MCL-1 localizes to the mitochondrial matrix and couples mitochondrial fusion to respiration." dans: Nature cell biology, Vol. 14, Issue 6, pp. 575-83, (2012) (PubMed).

Détails sur MCL-1

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Autre désignation: Mcl-1 (MCL1 Produits)

Synonymes: anticorps BCL2L3, anticorps EAT, anticorps MCL1-ES, anticorps MCL1L, anticorps MCL1S, anticorps Mcl-1, anticorps TM, anticorps bcl2-L-3, anticorps mcl1/EAT, anticorps AW556805, anticorps mcl-1, anticorps fb72f02, anticorps wu:fb70a08, anticorps wu:fb72f02, anticorps zfMLP1, anticorps zfMcl-1a, anticorps zgc:109835, anticorps zgc:109763, anticorps E3, anticorps E3-2, anticorps RNE3, anticorps RNU107, anticorps SNORA63A, anticorps elF-4AII, anticorps MCL1, BCL2 family apoptosis regulator, anticorps myeloid cell leukemia sequence 1, anticorps MCL1, BCL2 family apoptosis regulator a, anticorps MCL1, BCL2 family apoptosis regulator b, anticorps small nucleolar RNA, H/ACA box 63, anticorps MCL1, anticorps Mcl1, anticorps mcl1a, anticorps mcl1b, anticorps SNORA63

Sujet:

Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Gene Name: Mcl1

ID gène: 17210

UniProt: P97287

Pathways: Signalisation MAPK