MCL-1 anticorps (Internal Region)

N° du produit ABIN6657786

Détail du produit anti-MCL-1 anticorps

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Épitope: Internal Region

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCL-1 est non-conjugé

Application: Western Blotting (WB), ELISA

Purification: This affinity-purified antibody was prepared from monospecific antiserum by immunoaffinity chromatography using synthetic peptide coupled to agarose beads followed by cross adsorption to remove any unwanted reactivity.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Immunogène:

Immunogen: This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse Mcl-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This antibody is suitable for western blotting, immunoprecipitation, and ELISA.  This antibody detects mouse Mcl-1 and is not expected to cross react with the human sequence.  Cross reactivity with Mcl-1 from other sources is unknown.  This antibody is highly specific, showing no bands in null cell lysates from Mcl-1 knockout mice even when grossly over-exposed. Mouse Mcl-1 (myeloid cell leukemia sequence 1) is composed of 331 amino acids and is reported to be 35.2 kDa in size.  The human ortholog consists of 351 amino acids and is reported to be 37.3 kDa in size.

ELISA Dilution: 1:10,000 - 1:50,000

Western Blot Dilution: 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.01 % (w/v) Sodium Azide

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Références pour anticorps anti-MCL-1 (ABIN6657786)

Suzuki, Okamoto, Katoh, Sugiyama, Kusakabe, Tokunaga, Hirano, Miyata, Fukuhara, Ikawa, Satoh, Yoshio, Suzuki, Saijo, Huang, Kanto, Akira, Matsuura: "Infection with flaviviruses requires BCLXL for cell survival." dans: PLoS pathogens, Vol. 14, Issue 9, pp. e1007299, (2019) (PubMed).

Tuzlak, Schenk, Vasanthakumar, Preston, Haschka, Zotos, Kallies, Strasser, Villunger, Herold: "The BCL-2 pro-survival protein A1 is dispensable for T cell homeostasis on viral infection." dans: Cell death and differentiation, Vol. 24, Issue 3, pp. 523-533, (2018) (PubMed).

Wang, Ni, Dorko, Kumer, Schmitt, Nawabi, Komatsu, Huang, Ding: "Increased hepatic receptor interacting protein kinase 3 expression due to impaired proteasomal functions contributes to alcohol-induced steatosis and liver injury." dans: Oncotarget, Vol. 7, Issue 14, pp. 17681-98, (2017) (PubMed).

Goodall, Finch-Edmondson, van Vuuren, Yeoh, Gentle, Vince, Ekert, Vaux, Callus: "Cycloheximide Can Induce Bax/Bak Dependent Myeloid Cell Death Independently of Multiple BH3-Only Proteins." dans: PLoS ONE, Vol. 11, Issue 11, pp. e0164003, (2017) (PubMed).

Ohmer, Weber, Sutter, Ehrhardt, Zimmermann, Häcker: "Anti-apoptotic Bcl-XL but not Mcl-1 contributes to protection against virus-induced apoptosis." dans: Cell death & disease, Vol. 7, Issue 8, pp. e2340, (2017) (PubMed).

Singh, Roukounakis, Frank, Kirschnek, Das, Neumann, Madl, Römer, Zorzin, Borner, Haimovici, Garcia-Saez, Weber, Häcker: "Dynein light chain 1 induces assembly of large Bim complexes on mitochondria that stabilize Mcl-1 and regulate apoptosis." dans: Genes & development, Vol. 31, Issue 17, pp. 1754-1769, (2017) (PubMed).

Lee, Wales, Escudero, Cohen, Luccarelli, Gallagher, Cohen, Huhn, Bird, Engen, Walensky: "Allosteric inhibition of antiapoptotic MCL-1." dans: Nature structural & molecular biology, Vol. 23, Issue 6, pp. 600-7, (2017) (PubMed).

Sochalska, Schuler, Weiss, Prchal-Murphy, Sexl, Villunger: "MYC selects against reduced BCL2A1/A1 protein expression during B cell lymphomagenesis." dans: Oncogene, Vol. 36, Issue 15, pp. 2066-2073, (2017) (PubMed).

Omari, Waters, Naranian, Kim, Perumalsamy, Chi, Greenblatt, Moley, Opferman, Jurisicova: "Mcl-1 is a key regulator of the ovarian reserve." dans: Cell death & disease, Vol. 6, pp. e1755, (2016) (PubMed).

Thornton, Delgado, Chen, Salas, Krementsov, Fernandez, Vernia, Davis, Heimann, Teuscher, Krangel, Ramiro, Rincón: "Inactivation of nuclear GSK3β by Ser(389) phosphorylation promotes lymphocyte fitness during DNA double-strand break response." dans: Nature communications, Vol. 7, pp. 10553, (2016) (PubMed).

Sochalska, Ottina, Tuzlak, Herzog, Herold, Villunger: "Conditional knockdown of BCL2A1 reveals rate-limiting roles in BCR-dependent B-cell survival." dans: Cell death and differentiation, Vol. 23, Issue 4, pp. 628-39, (2016) (PubMed).

DOrsi, Engel, Pfeiffer, Nandi, Kaufmann, Henshall, Prehn: "Bok Is Not Pro-Apoptotic But Suppresses Poly ADP-Ribose Polymerase-Dependent Cell Death Pathways and Protects against Excitotoxic and Seizure-Induced Neuronal Injury." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 16, pp. 4564-78, (2016) (PubMed).

Vier, Groth, Sochalska, Kirschnek: "The anti-apoptotic Bcl-2 family protein A1/Bfl-1 regulates neutrophil survival and homeostasis and is controlled via PI3K and JAK/STAT signaling." dans: Cell death & disease, Vol. 7, pp. e2103, (2016) (PubMed).

Myasnikov, Kundhavai Natchiar, Nebout, Hazemann, Imbert, Khatter, Peyron, Klaholz: "Structure-function insights reveal the human ribosome as a cancer target for antibiotics." dans: Nature communications, Vol. 7, pp. 12856, (2016) (PubMed).

Chen, Kanai, Inoue-Yamauchi, Tu, Huang, Ren, Kim, Takeda, Reyna, Chan, Ganesan, Liao, Gavathiotis, Hsieh, Cheng: "An interconnected hierarchical model of cell death regulation by the BCL-2 family." dans: Nature cell biology, Vol. 17, Issue 10, pp. 1270-81, (2016) (PubMed).

Choi, Harhaj: "HTLV-1 tax stabilizes MCL-1 via TRAF6-dependent K63-linked polyubiquitination to promote cell survival and transformation." dans: PLoS pathogens, Vol. 10, Issue 10, pp. e1004458, (2015) (PubMed).

Ma, Nguyen, Tan, Ninnis, Iyer, Stroud, Menard, Kluck, Ryan, Dewson: "Bax targets mitochondria by distinct mechanisms before or during apoptotic cell death: a requirement for VDAC2 or Bak for efficient Bax apoptotic function." dans: Cell death and differentiation, Vol. 21, Issue 12, pp. 1925-35, (2015) (PubMed).

Williams, Ni, Haynes, Manley, Li, Jaeschke, Ding: "Chronic Deletion and Acute Knockdown of Parkin Have Differential Responses to Acetaminophen-induced Mitophagy and Liver Injury in Mice." dans: The Journal of biological chemistry, Vol. 290, Issue 17, pp. 10934-46, (2015) (PubMed).

Rutherford, Childs, Ohotski, McGlynn, Riddick, MacFarlane, Tasker, Pyne, Pyne, Edwards, Palmer: "Regulation of cell survival by sphingosine-1-phosphate receptor S1P1 via reciprocal ERK-dependent suppression of Bim and PI-3-kinase/protein kinase C-mediated upregulation of Mcl-1." dans: Cell death & disease, Vol. 4, pp. e927, (2014) (PubMed).

Selimoglu-Buet, Gallais, Denis, Guillouf, Moreau-Gachelin: "Oncogenic kit triggers Shp2/Erk1/2 pathway to down-regulate the pro-apoptotic protein Bim and to promote apoptosis resistance in leukemic cells." dans: PLoS ONE, Vol. 7, Issue 11, pp. e49052, (2013) (PubMed).

Détails sur MCL-1

Antigène: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Autre désignation: Mcl-1 (MCL1 Produits)

Synonymes: anticorps BCL2L3, anticorps EAT, anticorps MCL1-ES, anticorps MCL1L, anticorps MCL1S, anticorps Mcl-1, anticorps TM, anticorps bcl2-L-3, anticorps mcl1/EAT, anticorps AW556805, anticorps mcl-1, anticorps fb72f02, anticorps wu:fb70a08, anticorps wu:fb72f02, anticorps zfMLP1, anticorps zfMcl-1a, anticorps zgc:109835, anticorps zgc:109763, anticorps E3, anticorps E3-2, anticorps RNE3, anticorps RNU107, anticorps SNORA63A, anticorps elF-4AII, anticorps MCL1, BCL2 family apoptosis regulator, anticorps myeloid cell leukemia sequence 1, anticorps MCL1, BCL2 family apoptosis regulator a, anticorps MCL1, BCL2 family apoptosis regulator b, anticorps small nucleolar RNA, H/ACA box 63, anticorps MCL1, anticorps Mcl1, anticorps mcl1a, anticorps mcl1b, anticorps SNORA63

Sujet:

Synonyms: rabbit anti-Mcl-1 antibody, Mcl1, Mcl 1, Bcl 2 related protein EAT/mcl1 antibody, Bcl2 related antibody, EAT antibody, Induced myeloid leukemia cell differentiation protein Mcl-1 antibody

Background: Regulated apoptosis is essential for both the development and the subsequent maintenance of the immune system. Interleukins, including IL-2, IL-4, IL-7 and IL-15, heavily influence lymphocyte survival during the vulnerable stages of VDJ rearrangement and later in ensuring cellular homeostasis, but the genes specifically responsible for the development and maintenance of lymphocytes have not been identified. The Anti apoptotic protein Mcl-1 (myeloid cell leukemia sequence 1 (BCL2-related)) is an attractive candidate, as it is highly regulated, appears to enhance short-term survival and functions at an apical step in genotoxic deaths. However, Mcl-1 deficiency results in peri-implantation lethality. Mice, conditional for Mcl-1, display a profound reduction in B and T lymphocytes when Mcl-1 is removed. Deletion of Mcl-1 during early lymphocyte differentiation increases apoptosis and arrests the development at pro-B-cell and double negative T-cell stages. Induced deletion of Mcl-1 in peripheral B- and T-cell populations results in their rapid loss. Moreover, IL-7 both induces and requires Mcl-1 to mediate lymphocyte survival. Mcl-1 is essential both early in lymphoid development and later on in the maintenance of mature lymphocytes.

Gene Name: Mcl1

ID gène: 17210

UniProt: P97287

Pathways: Signalisation MAPK