CD11b anticorps (PE)

N° du produit ABIN6657853

N° du produit (Fournisseur): 200-508-n79

L’anticorps Rat Monoclonal anti-CD11b a été validé pour FACS, IHC, IP, IF, FM et MA. Il convient pour détecter CD11b dans des échantillons de Souris.

Aperçu rapide pour CD11b anticorps (PE) (ABIN6657853)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Clone: M1-70

Détail du produit anti-CD11b anticorps (PE)

N° du produit (Fournisseur): 200-508-n79

Fournisseur: Rockland

Fonction: CD11b Phycoerythrin Antibody

Réactivité croisée (Details): Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine).

Purification: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b.

Stérilité: Sterile filtered

Immunogène: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotype: IgG2b kappa

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: 10 μL/10^6 cells (0.1 μg)

IF_Microscopy_Dilution: 0.25 μg/10x6 cells

Commentaires:

Anti-CD11b is tested for FLOW and IF, and useful for Immunoprecipitation and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Date de péremption: 6 months

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b

Sujet:

Synonyms: CD11b

Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gene Name: Itgam

UniProt: A1E2I0

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation