CD11b anticorps (PE)

N° du produit ABIN6657853

Détail du produit anti-CD11b anticorps (PE)

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD11b est conjugé à/à la PE

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Réactivité croisée (Details): Cross reactivity Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)

Purification: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD11b. . Cross-reactivity with CD11b from other sources has not been tested. Anti-CD11b is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Immunogène:

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Clone: M1-70

Isotype: IgG2b

Labeling Ratio: 1-2

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD11b is useful for Immunoprecipitation, Immunohistochemistry, and Flow Cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

IF Microscopy Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

0.09 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Détails sur CD11b

Antigène: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Autre désignation: CD11b (ITGAM Produits)

Synonymes: anticorps CD11b/CD18, anticorps CR3, anticorps CR3A, anticorps Cd11b, anticorps F730045J24Rik, anticorps Ly-40, anticorps MAC1, anticorps Mac-1, anticorps Mac-1a, anticorps CD11B, anticorps MAC-1, anticorps MAC1A, anticorps MO1A, anticorps SLEB6, anticorps C3bi, anticorps integrin subunit alpha M, anticorps integrin alpha M, anticorps integrin, alpha M (complement component 3 receptor 3 subunit), anticorps integrin alpha-M, anticorps ITGAM, anticorps Itgam, anticorps LOC100351865

Sujet:

Synonyms: CD11b

Background: CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Gene Name: Itgam

UniProt: A1E2I0

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation