Chèvre anti-Souris IgG (Fc Region) Anticorps (DyLight 800) - Preadsorbed
-
- Antigène Tous les produits IgG
- IgG
-
Épitope
- Fc Region
-
Reactivité
- Souris
-
Hôte
- Chèvre
-
Clonalité
- Polyclonal
-
Conjugué
- DyLight 800
-
Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Attributs du produit
-
Synonyms: Goat Anti Mouse IgG F(c) Antibody DyLight™ 800 Conjugated, Goat Anti-Mouse IgG Fc Antibody DyLight™ 800 Conjugated, Goat Anti Mouse IgG Fc Fragment Antibody DyLight™ 800 Conjugated
Background: Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
-
Immunogen: Mouse IgG F(c) fragment
- Isotype
- IgG
- Labeling Ratio
- 2.3
-
-
- Indications d'application
-
Application Note: The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
-
-
Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." dans: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).
: "
-
Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." dans: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).
-
- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
-